Carmine-Fibrin
Historical document, translated for reference. It reflects medical knowledge of the 1920s–30s and is not medical advice.
Summary
Carmine-fibrin is a reagent proposed by Grutzner in 1875 for qualitative and quantitative determination of pepsin in gastric juice. It involves using fibrin stained with carmine to detect pepsin activity through the release of carmin when the fibrin is dissolved by gastric juice.
Encyclopedia entry (1928–1936)
CARMINE-FIBRIN, proposed by Grutzner in 1875 for qualitative and quantitative determination of pepsin in gastric juice. Well-washed with water fibrin (until completely decolorized) is cut into small pieces and stained for 1-2 days in a weak ammoniacal 1/i-% solution of carmine. After thorough washing with water (until the wash water is colorless), the stained fibrin is pressed and stored in glycerin. To perform the reaction, the fibrin is washed from glycerin, an equal volume of 0.1% hydrochloric acid (2-3 drops) is poured onto it, and 5 cm3 of gastric juice filtrate is added. They are mixed and placed in an incubator for 6-12 hours. When the fibrin dissolves under the influence of gastric juice, carmine is released, which colors the liquid.
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Cite this page
“Carmine-Fibrin.” Soviet Medical Encyclopedia. English translation of Bolshaya Meditsinskaya Entsiklopediya, 1st ed. (Moscow, 1928–1936), ed. N. A. Semashko. https://sovietmedicalencyclopedia.pages.dev/article/carmine-fibrin/