Dominici Methods

DOMINICI METHODS (Dominici). A. Dominici staining of histological preparations—sections are stained for 20-30 minutes in solution I (0.5 g eosin, 0.6 g Orange G in 100 cm³ of distilled water), rinsed in 60% alcohol, and restained for 1-2 minutes in solution II (0.5 g toluidine blue in 100 cm³ of distilled water), after which they are differentiated in 96% alcohol until the paint clouds cease to detach, dehydrated in absolute alcohol, and mounted in Canadian balsam. The results are blue nuclei, red erythrocytes, and orange connective tissue fibers. In a modification of this method proposed by Tishutkin, instead of solution I, either a mixture consisting of 10 cm³ of filtered and twice-diluted concentrated Orange G solution and 2 cm³ of concentrated iodine solution in absolute alcohol, or an equal mixture of concentrated Orange G and Fuchsin S solutions is used. The staining is usually applied after mercuric chloride, picric, formalin, and alcohol fixatives and provides a fine histological differentiation of complex tissues; it is very suitable for staining sections of blood-forming organs. B. Dominici fixation—20 parts of a concentrated aqueous mercuric chloride solution are mixed with 2 parts of iodine tincture, filtered, and 2 parts of formalin are added to the filtrate. Fix for 1 hour; it is used for fixing various tissues, especially the intestine.