Dungern Modification

DUNGERN MODIFICATION (von Dun-gern) of the Wassermann reaction, represents a simplification of the Noguchi method. As antigen, Dungern uses an alcoholic extract of guinea pig heart; complement is guinea pig serum dried on filter paper; the hemolytic system consists of rabbit hemolytic serum immunized with human erythrocytes; the hemolytic serum is also used in dry form (on filter paper). Instead of the test subject's inactivated serum, defibrinated blood of the person being examined is used, in which there is therefore active Z-serum (--human erythrocytes (2nd component of the hemolytic system).--Procedure: take two test tubes and pour 2 cm³ of NaCl into each; then add a piece of filter paper with dried complement and 0.1 cm³ of defibrinated blood of the test subject (the blood is defibrinated with a match on a watch glass) and into one test tube also add one drop of antigen; leave for 1 hour at room temperature, then add filter paper with dried hemolytic amboceptor. If in the test tube to which antigen was added, there is a delay in hemolysis, and in the other + hemolysis, then the reaction is positive; if in both there is hemolysis, the reaction is negative; the method met sharp criticism from Plaut, Meyer and others; its supporter is Kepinov.