Huppert Reaction
Historical document, translated for reference. It reflects medical knowledge of the 1920s–30s and is not medical advice.
Summary
The Huppert reaction, often used in Nakayama's modification, detects minute amounts of bile pigments. It is particularly useful when urine contains significant amounts of indican and other urinary pigments. The test involves mixing urine with a barium chloride solution, centrifuging, and then adding a specific reagent. The resulting color change, which intensifies upon heating, indicates the presence of bile pigments at a concentration of 1:200,000 parts of urine.
Encyclopedia entry (1928–1936)
HUPPERT REACTION (Huppert), usually applied in Nakayama's modification, detects minute amounts of bile pigments; it is especially applicable when the urine contains a significant amount of indican and urinary pigments. 6 cm3 of urine is mixed with an equal amount of a 10% aqueous solution of barium chloride. The mixture is centrifuged, the clear layer is decanted, and 3-4 cm3 of reagent consisting of 99 parts of 95% alcohol and 1 part of fuming HN03 is added to the precipitate. The mixture takes on a green or bluish-green color, especially clear upon heating. With the addition of 1 drop of fuming HN03, a bluish, violet, and red color is obtained. The reaction detects bile pigments at a content of 1:200,000 parts of urine.
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Cite this page
“Huppert Reaction.” Soviet Medical Encyclopedia. English translation of Bolshaya Meditsinskaya Entsiklopediya, 1st ed. (Moscow, 1928–1936), ed. N. A. Semashko. https://sovietmedicalencyclopedia.pages.dev/article/huppert-reaction/