Holzer Method

HOlZER METHOD (Helzer) is used for staining glial fibers and has almost completely replaced the older methods, including Weigert's. Its advantages: it can be applied to any formalin-fixed material, including frozen sections, requires only 10-15 minutes, is selective, although it also stains astrocytes. Technique: formalin fixation of sections made on a freezing microtome; the sections are transferred to a glass slide with a glass rod into the following solution: ¹/_a% phosphomolybdic acid - 1 hour, 96% or absolute alcohol - 2 hours, keep for 30-60-90 seconds. Then the sections are transferred to clean, defatted glass, allowing the liquid to drain; wipe the glass around the section with a cloth and remove excess moisture 2-3 times with filter paper moistened with alcohol-chloroform (2 cm³ alcohol to 8 cm³ chloroform). The sections should adhere to the glass; to prevent the section from drying, work quickly and pour the following stain onto the moist section: absolute alcohol - 2 cm³, chloroform - 8 cm³, crystal violet - 0.5 g. - Next, quickly pour a 10% solution of potassium bromide onto the stained section at an angle, without letting the stain thicken (work quickly). The stain comes off in layers. Try to remove the greenish bromine deposits so that the section, freed from them, appears uniformly stained, black-violet. - Wipe the stain thoroughly around the section and blot once with filter paper moistened with a liquid of the following composition: aniline oil - 4 cm³, chloroform - 6 cm³, 1% hydrochloric acid - 1 drop. If cloudy, filter. Differentiate with the aforementioned liquid until the section is light lilac. Xylene, balsam.

For successful staining, work quickly and with freshly prepared reagents. The stain thickens quickly and can therefore leave persistent deposits, so add absolute alcohol with chloroform (2:8) to the old solution, and if necessary, filter the stain. Do not overuse the differentiator, but rather proceed to pure xylene. For fixation, initially take an equal mixture of 96% alcohol and 10% formalin (8 days), then place the piece in 10% formalin for two days before sectioning. For celloidin sections, remove the celloidin from the glass with methyl alcohol, then press by blotting with filter paper and proceed as described above. p. Snesarev. KOLTSOV Boris Nikolaevich (born in 1861), a leading urologist. Graduated from the natural sciences faculty and the Military Medical Academy. He worked in surgery under Kolominin and Troyanov, and in urology under Kaspar. In 1901, having been put in charge of an independent department at the Obukhovskaya Hospital, he began to cultivate urology there, founding the first specialized urological hospital in Petersburg. Currently, he is a professor at the Department of Urology of the State Institute for Advanced Medical Training in Leningrad, heads the urology department of the Hospital in Memory of the Victims of the Revolution, is an honorary member and chairman of the Leningrad Society of Urologists, and a member of the Leningrad Soviet. The main feature of K.'s activity is the close connection between practical hospital work and the theoretical scientific and experimental development of various issues in urology. K. is an extremely popular urologist in the Union, as a significant number of doctors have passed through his department at the institute. K. is co-editor of the journals 'Urology' and 'Zeitschrift f. urolog. Chirurgie'. His literary work consists of 70 printed works, including several monographs and textbooks. The most important are 'Injuries and diseases of the prostate gland' (Russ. Khir., vol. 9, 1909), 'Diagnosis of diseases of the genitourinary organs' (SPB, 1911), 'Guide to Urology' (M.-L., 1924), 'General and Private Urology' (vols. 1-7, L., 1924-28), 'Gonorrhea in men and women' (a very popular textbook that went through 3 editions), works on sexual tuberculosis, on the surgical treatment of strictures and fistulas of the urethra, etc.