Pantin Method
Historical document, translated for reference. It reflects medical knowledge of the 1920s–30s and is not medical advice.
Summary
The Pantin Method is an auxiliary technique for colorimetric determination of pH in microscopically small objects. It involves projecting images of colored standard tubes into the microscope field of view to compare the color of an indicator introduced into cellular structures with simultaneously visible buffer solutions of known pH.
Encyclopedia entry (1928–1936)
PANTIN METHOD (Pantin, 1923), an auxiliary method used for colorimetric determination of pH in microscopically small objects. The distinctive feature of this method is that the image of a series of colored standard tubes is projected into the field of view of the microscope, whereby the color or shade that a given indicator has acquired, for example when introduced into the nucleus or cytoplasm of a cell by means of a micropipette (see Microsurgery), can be compared with the color of the simultaneously visible buffer standard solutions (with known pH) in the same field of view, stained with the same indicator, and in this way determine the pH (see also Hydrogen ions, and Indicator method).
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Cite this page
“Pantin Method.” Soviet Medical Encyclopedia. English translation of Bolshaya Meditsinskaya Entsiklopediya, 1st ed. (Moscow, 1928–1936), ed. N. A. Semashko. https://sovietmedicalencyclopedia.pages.dev/article/pantin-method/