Trichrome Staining
Historical document, translated for reference. It reflects medical knowledge of the 1920s–30s and is not medical advice.
Summary
Trichrome staining is a histological staining method recommended for tissue sections fixed with mercuric chloride. The procedure involves multiple steps with specific reagents to stain different cellular components in distinct colors.
Encyclopedia entry (1928–1936)
Trichrome staining is recommended for staining histological sections after mercuric chloride fixation. Recipe: mordant in a 1% aqueous solution of resorcinol for two hours; washing with water, then 10-20 minutes in a 1% aqueous solution of acridine; after rapid washing with water for 2 minutes, picric acid-violet blue (5 cm³ of 1% aqueous solution of violet blue and 95 cm³ of saturated aqueous solution of picric acid); short wash in water, rapid dehydration and mounting in Canadian balsam. Nuclei and colloid (of the thyroid gland) are red, protoplasm is green, the brush border (Burstenbesatz) is blue, connective tissue is light blue, erythrocytes are yellow.
Cite this page
“Trichrome Staining.” Soviet Medical Encyclopedia. English translation of Bolshaya Meditsinskaya Entsiklopediya, 1st ed. (Moscow, 1928–1936), ed. N. A. Semashko. https://sovietmedicalencyclopedia.pages.dev/article/trichrome-staining/