Bogomolov's Reaction
Historical document, translated for reference. It reflects medical knowledge of the 1920s–30s and is not medical advice.
Summary
Bogomolov's reaction is a historical chemical test used to detect the presence of urobilin in urine. The procedure involves adding copper sulfate and chloroform to a urine sample, where a reddish coloration of the chloroform layer indicates a high concentration of urobilin.
Encyclopedia entry (1928–1936)
BOGOMOLOV'S REACTION serves to detect urobilin in urine. To 10 cubic centimeters of urine, 6–8 drops of a copper sulfate solution and 1–2 cubic centimeters of chloroform are added. In the presence of a large quantity of urobilin, the chloroform, after shaking and allowing the mixture to settle, becomes colored a reddish hue.
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“Bogomolov's Reaction.” Soviet Medical Encyclopedia. English translation of Bolshaya Meditsinskaya Entsiklopediya, 1st ed. (Moscow, 1928–1936), ed. N. A. Semashko. https://sovietmedicalencyclopedia.pages.dev/article/bogomolovs-reaction/