Janus Green B

Janus Green B and G (Hochst), a basic safranine-azo dye (C.H₅)_SN-N = -N(CH₃),Cl. A brown or dark green powder. Easily soluble in water with a blue-green color. Introduced into microtechnique by Ehrlich. Stains mitochondria in living cells during life. Usually used in a dilution of 1:10,000-50,000 in physiological solution, Ringer's solution, or Locke's solution. Staining usually occurs in the presence of oxygen. A strong protoplasmic poison (1:200,000). Staining of blood cells is done by directly adding a drop of the dye to a drop of blood, after which the mixture is covered with a cover glass. The staining lasts 1-2 hours. In a mixture of J. with Neutralrot, the mitochondria are stained green at the same time, while various secretory granules and elements of the Golgi apparatus are stained red.

In a mixture of J. with Neutralrot, the mitochondria are stained green at the same time, while various secretory granules and elements of the Golgi apparatus are stained red.